DNA Primer Design For Polymerase Chain Reaction
Tips & Tricks- What your mentor never taught you
"My Students' Testimonials"
"This was very helpful! A lot of mentors only teach you all the steps you need to take in order to have a successful PCR, but often these details are omitted. I believe the real success is in learning: 1) why we take certain steps? 2) why does it work (or does not)? 3) why am I doing this? This is definitely a tool I will be using with my undergrads. Thank you for taking the time to design this course!"
-Dr. Edwin, Wisconsin University
"I must congratulate you for taking this initiative and sharing the practical knowledge to fellow students working in the area of life science. You really designed a nice course with easy to follow content. The course is nicely following the easy to complex approach. There are enough graphics used to share the knowledge in interesting way, and whatever required information is provided"
-Rakesh, PhD., Tohoku University, Japan
"This course provided me with the essential fundamentals of primer design. it's fun and easy to learn. I'd definitely recommend it for students interested. Looking forward to the new series. Thank you!"
-Farah, MSc., American University of Beirut
"I am getting so much out of it myself. The school helped me a lot with learning about the basics of primer design in a clear and simple way. I trust that the information provided will be very helpful for students and anyone working in biology sector. Well done!"
- Asma', National Center for Diabetes Endocrinology and Genetics, Jordan
We are on Udemy now
https://www.udemy.com/course/dna-primer-design-for-pcr/
Why this is important for you?
Well, have you ever gotten a nonspecific band in your gel electrophoresis runs? Or a band with a different size than your target DNA? I'm betting the answer is yes. Who knew that a DNA primer sequence could cause so much trouble?
No worries! When it comes to polymerase chain reaction (PCR) primer design we all have some sort of weakness.
With this course, you won't have to worry about any difficulties any more.
This is the first online school that explains all the criteria for primer design in detail. We bring it all to you in one place!!
Levels of primary target audience
Undergraduate students
Early career researchers
Preview Three lectures here..
Class curriculum includes:
Detailed explanation of PCR technique and PCR primer design criteria with instructor voice.
Real example on how to design a PCR primer step by step using required programs; NCBI, Primer3, BLAT, delta g analysis, and secondary structure analysis, with instructor voice.
Total Lectures
23
Language
English
Includes
Lifetime access
Course Curriculum
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StartLecture 1. List of the PCR Primer Design Criteria + Definition of Primer Specificity and Sensitivity (1:44)
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StartLecture 2. The DNA Primer Length (2:54)
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StartLecture 3. Primer Melting Temperature (Tm)-Wallace equation, Marmmur & Doty equation, and the Salt-adjusted method (3:56)
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StartLecture 4. How to Calculate Enthalpy, Entropy, and Free Energy (5:25)
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StartLecture 5. Primer Tm-The Nearest Neighbor Method (2:54)
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StartLecture 6. Annealing Temperature (Ta) of the DNA Primer (3:02)
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StartLecture 7. GC-Content of the DNA Primer (2:30)
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StartLecture 8. GC Clamp at the 3’ End of the DNA Primer (4:01)
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StartLecture 9. Primer Secondary Structure (5:25)
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StartLecture 10. Runs & Repeats in the primer (2:54)
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StartLecture 11. Cross Homology
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StartLecture 12. Primer Product Length
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StartLecture 1. Retrieve a Gene Sequence form NCBI, and Determine the Exact Location for Each Exon on the Chromosome Using Graphics (6:13)
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Startlecture 2. Compare Different mRNA Transcripts and Select One to Evaluate a Gene Expression in a Novel Cells (4:56)
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StartLecture 3. Understand Primer3 Setting (4:39)
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StartLecture 4 Calculate Primer Self-Complementarity Score
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StartLecture 5. Check for Primer Cross Homology Using BLAT (2:28)
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StartLecture 6. Evaluate Primers Depending on Delta G (4:09)
This course is closed for enrollment.
Reviews
Its good and short course for beginning students thanx for this skills!
Israa
I found the tutelage simplified enough to understand not just the theoretical basis of PCR, but to appreciate the experimental know-how in preparation for a real time laboratory engagement. This is an excellent online presentation that should appeal across board!!!
Michael
It's very helpful. Thank you so much
Valeria
Thank you very much, its useful lecture
Media
It is very helpful. lectures are good. They help a lot
DEEPTI
This was very helpful! A lot of mentors only teach you all the steps you need to take in order to have a successful PCR, but often these details are omitted. I believe the real success is in learning: 1) why we take certain steps? 2) why does it work (or does not)? 3) why am I doing this? This is definitely a tool I will be using with my undergrads. Thank you for taking the time to design this course!
Edwin
Elaborate research simplified. Keep up the good work!!! This is an intelligent presentation!!!
Michael
