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DNA Primer Design For Polymerase Chain Reaction
Polymerase Chain Reaction (PCR)
Lecture 1. PCR Cycling (3:06)
Lecture 2. The Standard Conditions for the PCR amplification program + Exponential Amplification (3:10)
Lecture 3. The size difference between the PCR amplification products of the 1st, 2nd and 3rd PCR cycle (2:57)
PCR Primer Design Criteria
Lecture 1. List of the PCR Primer Design Criteria + Definition of Primer Specificity and Sensitivity (1:44)
Lecture 2. The DNA Primer Length (2:54)
Lecture 3. Primer Melting Temperature (Tm)-Wallace equation, Marmmur & Doty equation, and the Salt-adjusted method (3:56)
Lecture 4. How to Calculate Enthalpy, Entropy, and Free Energy (5:25)
Lecture 5. Primer Tm-The Nearest Neighbor Method (2:54)
Lecture 6. Annealing Temperature (Ta) of the DNA Primer (3:02)
Lecture 7. GC-Content of the DNA Primer (2:30)
Lecture 8. GC Clamp at the 3’ End of the DNA Primer (4:01)
Lecture 9. Primer Secondary Structure (5:25)
Lecture 10. Runs & Repeats in the primer (2:54)
Lecture 11. Cross Homology
Lecture 12. Primer Product Length
Tools and Methods (How to...)
Lecture 1. Retrieve a Gene Sequence form NCBI, and Determine the Exact Location for Each Exon on the Chromosome Using Graphics (6:13)
lecture 2. Compare Different mRNA Transcripts and Select One to Evaluate a Gene Expression in a Novel Cells (4:56)
Lecture 3. Understand Primer3 Setting (4:39)
Lecture 4 Calculate Primer Self-Complementarity Score
Lecture 5. Check for Primer Cross Homology Using BLAT (2:28)
Lecture 6. Evaluate Primers Depending on Delta G (4:09)
Gel Electrophoresis Troubleshooting Directory
Lecture 1. Low Intensity of All or Some DNA Bands
Lecture 2. Smeared DNA Bands
Quiz
Quiz
Quiz Answers
Lecture 12. Primer Product Length
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